Lipopolysaccharide Induces the Rapid Tyrosine Phosphorylation of the Mitogen-Activated Protein Kinases erk-l and p38 in Cultured Human Vascular Endothelial Cells Requiring the Presence of Soluble CD14
نویسندگان
چکیده
Human vascular endothelial cells (HUVECsl, which do not display the lipopolysaccharide (LPS) receptor CD14, were examined for protein tyrosine phosphorylation after LPS stimulation in the presence and absence of soluble CD14 (sCD14). By phosphotyrosine Western blotting and immunocomplex kinase assays we show that LPS was capable of inducing in these cells rapid protein tyrosine phosphorylation and kinase activation of two members of the mitogenactivated protein kinase (MAPKI family erk-l and the newly discovered p38, requiring the presence of sCD14. LPS-induced tyrosine phosphorylation of MAPK was associated
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